Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

TUN Yun-Chao; CA Yi-Quan

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August 1993

TUN Yun-Chao and CA Yi-Quan. Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe[J]. Virologica Sinica, 1993, 8(4): 310-315.

Citation: TUN Yun-Chao, CA Yi-Quan. Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe .VIROLOGICA SINICA, 1993, 8(4) : 310-315.

HBV-DNA的生物素寡聚核苷酸探针快速检测

吴云涛 ,
蔡宜权

1.
中国科学院武汉病毒研究所武汉

摘要

本文建立一种快速HBV-DNA检测方法。此法是基于生物素标记的寡聚核苷酸与硝酸纤维素滤膜上的靶DNA杂交,然后通过Streptoavidin-碱性磷酸酶偶联体及生色底物进行检测。本文研究了此法的实用性,表明对于实验室及临床的HBV-DNA检测,此法是一种快速(4小时)、灵敏(110pg)、特异、方便的方法。
- HBV-DNA
- , DNA杂交
- , 生物素寡聚核苷酸探针

Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

TUN Yun-Chao ,
CA Yi-Quan

1.
Wuhan Institute of Virology

Abstract

A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.
- HBV-DNA
- , DNA Hybridization
- , Biotinylated oligonucleotide

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

TUN Yun-Chao ,
CA Yi-Quan

1. Wuhan Institute of Virology

Abstract: A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.