Study on expression of Epstein-Barr virus nuclear antigens in various infected states of cells

ZHAN De-Jin; CHEN Jian-Jing

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February 1994

ZHAN De-Jin and CHEN Jian-Jing. Study on expression of Epstein-Barr virus nuclear antigens in various infected states of cells[J]. Virologica Sinica, 1994, 9(1).

Citation: ZHAN De-Jin, CHEN Jian-Jing. Study on expression of Epstein-Barr virus nuclear antigens in various infected states of cells .VIROLOGICA SINICA, 1994, 9(1) : 13.

不同感染状态下EB病毒核抗原亚型表达的研究

詹德进 ,
陈剑经

1.
广州医学院化学致癌研究所中山医科大学肿瘤研究所

摘要

本实验用免疫印迹法纯化的抗ＥＢＮＡ亚型抗体，结合显微荧光分光光度检测技术，检测在不同感染状态下，三种亚型ＥＢＮＡ抗原在细胞中的表达程度。结果表明，处于潜伏感染状态下的Ｒａｊｉ细胞ＥＢＮＡ－１表达量较大，经巴豆油和正丁酸诱导进入钝挫感染状态后ＥＢＮＡ－１表达减少，而ＥＢＮＡ－２的表达增强。Ｂ＿（９５－８）细胞也有相似的趋势。表明ＥＢ病毒的激活可能与不同ＥＢＮＡ亚型表达量的改变有关。
- EB病毒核抗原免疫印迹抗体纯化抗原定量

Study on expression of Epstein-Barr virus nuclear antigens in various infected states of cells

1.
Institute for Chemical Carcinagenesis,Guangzhou Medical College,Guangzhou 510182

Abstract

y microspectrophotometer quantitation(the expressing level of three kinds of EBNAs were detected with anti￣EBNA antitheies purified from human serum by Western blotting method,The resultsshowed that EBNA-1 expressed greatly in latently infected Rajicell.When they were got into aborted-ly infected state treated with croton oil and n-Butyrate,the ex pression level of EBNA-1 decreased andthat of EBAN-2 increased,B_(95-8) cell had a similar variation,It suggeets that the activation of EB virusmight associated with the expression level of EBNAs.
- Epetein-Bair virus nuclear antigen（EBNA） Immunoblotting Antibody purification Antigen quantitaton

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on expression of Epstein-Barr virus nuclear antigens in various infected states of cells

1. Institute for Chemical Carcinagenesis,Guangzhou Medical College,Guangzhou 510182

Abstract: y microspectrophotometer quantitation(the expressing level of three kinds of EBNAs were detected with anti￣EBNA antitheies purified from human serum by Western blotting method,The resultsshowed that EBNA-1 expressed greatly in latently infected Rajicell.When they were got into aborted-ly infected state treated with croton oil and n-Butyrate,the ex pression level of EBNA-1 decreased andthat of EBAN-2 increased,B_(95-8) cell had a similar variation,It suggeets that the activation of EB virusmight associated with the expression level of EBNAs.