Expression of HCV Core Protein in E．c0fi and Its Application

LI Yue-Xi; TANG Jia-Qi; SHI Jiang; ZHAO Hua-Zhong; JIAO Ren-Liang

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February 1994

LI Yue-Xi, TANG Jia-Qi, SHI Jiang, ZHAO Hua-Zhong and JIAO Ren-Liang. Expression of HCV Core Protein in E．c0fi and Its Application[J]. Virologica Sinica, 1994, 9(1).

Citation: LI Yue-Xi, TANG Jia-Qi, SHI Jiang, ZHAO Hua-Zhong, JIAO Ren-Liang. Expression of HCV Core Protein in E．c0fi and Its Application .VIROLOGICA SINICA, 1994, 9(1) : 18.

丙型肝炎病毒核心蛋白基因在大肠杆菌内的表达及应用

1.
南京军区军事医学研究所

摘要

将从中国丙肝病人血清中扩增克隆的丙型肝炎病毒核心蛋白基因（４０８ｂｐ）酶切处理后插入表达载体ｐＪＬＡ５０２内，获得高表达核心蛋白的重组工程菌。将重组菌经４２℃热诱导５ｈ，ＳＤＳ－ＰＡＧＥ分析表明，表达的核心蛋白占菌体蛋白总量的２０％。经分子筛和吸附层析纯化后获得的核心蛋白，ＥＬＩＳＡ检测证实有较好的抗原性和特异性。用表达的核心抗原加用表达的ＮＳ＿３抗原（Ｃ＿３３）装配的抗－ＨＣＶ试剂盒，经用标准血清验证及与国外第二代抗－ＨＣＶ试剂盒比较，证实符合丙肝诊断试剂要求。
- 丙型肝炎病毒核心蛋白基因表达大肠杆菌抗原性抗－HCV检测

Expression of HCV Core Protein in E．c0fi and Its Application

1.
Nanjing Military Medical Research Institute,Nanjing 210002

Abstract

The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.
- HCV Core Protein gene expression E. coli Antigen Anti-HCV detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of HCV Core Protein in E．c0fi and Its Application

1. Nanjing Military Medical Research Institute,Nanjing 210002

Abstract: The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.