TIAN Hua-Song, ZHANG Li-Ren, CHEN Li-Hua and CHEN Yao-Shun. The Fine Slrueture and Some Biophysical and BiochemicalCharacteristics of a Cytoplasmic.Polyhedrosis Virus fromLym antria xylina Swinhoe[J]. Virologica Sinica, 1995, 10(1).
Citation: TIAN Hua-Song, ZHANG Li-Ren, CHEN Li-Hua, CHEN Yao-Shun. The Fine Slrueture and Some Biophysical and Biochemical Characteristics of a Cytoplasmic.Polyhedrosis Virus from Lym antria xylina Swinhoe .VIROLOGICA SINICA, 1995, 10(1) : 67.

木毒蛾质型多角体病毒形态结构及理化特性的研究

  • 木毒蛾质型多角体病毒呈不规则多面体,大小差异较大,大多数在0.8~2.1μm之间;病毒粒子在多角体内随机分布,病毒粒子为正二十面体结构,有12个顶体,每个顶体上均有一个球状结构,平均直径约为60nm。经SDS-PAGE分析,多角体蛋白由一条分子量为3lkD的主带及3条次带组成;病毒粒子结构蛋白由6条多肽组成,分子量在12.6~33kD之间。多用体蛋白富含天冬氨酸,谷氨酸及酪氨酸,而半胱氨酸和甲巯氨酸含量较少,其碱性氨基酸与酸性氨基酸之比为1.43。病毒核酸对RNasc和DNase的稳定性试验及对甲醛的稳定性试验和吖啶橙染色试验,证明孩核酸为双链RNA,其解链温度为87℃,由10个基因片段组成,分子量在2.46×106~0.35×106道尔顿之间,总分子量为14.42×106道尔顿,电镜分析研究显示该核酸长度在0.5μm、1.0μm和1.3μm处各有一个分布峰。

The Fine Slrueture and Some Biophysical and Biochemical Characteristics of a Cytoplasmic.Polyhedrosis Virus from Lym antria xylina Swinhoe

  • The Lymantria,xylina Swinhoe cytoplasmic polyhedra xhows a quite difference ranging from 0.8 to2.lμm in diameter.The virions were randomly distributed in a polyhedron;The virions show icosahedron, and about 60 nm in diameter with l2 spikes.By the analysis of SDS-PAG E,the polyhedral pro-teins contain one major band(31000 Dalton)and three minor tsands.The virlon has 6 polypoptidesranging from 12600 to 33000 Daltons,The protein from LxCPV is rich in aslxirtic acid,glutamic acidand tyrosine,but poorer in cystein and methionine; The ratio of basic amino to acidic amino is l.43.By the stability test of the LxCPV RNA to RNase ,DNase and formaldehyde,the staining reaction ofacridine orange,the RNA is demontrated as double-strand RNA(dsRNA)with a Tm of 87℃.TheRNA genome consists of ten molecular mass from 2.46 to 0.35 MD,and with a totol estimated moleeular mass of l4.42 MD,Three distributive peaks of LxCPV RNA segments contour length are foundat 0.5μm,1.0μm and l.3μm respectlvely by electron micrcacopic analysis.

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    The Fine Slrueture and Some Biophysical and Biochemical Characteristics of a Cytoplasmic.Polyhedrosis Virus from Lym antria xylina Swinhoe

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    Abstract: The Lymantria,xylina Swinhoe cytoplasmic polyhedra xhows a quite difference ranging from 0.8 to2.lμm in diameter.The virions were randomly distributed in a polyhedron;The virions show icosahedron, and about 60 nm in diameter with l2 spikes.By the analysis of SDS-PAG E,the polyhedral pro-teins contain one major band(31000 Dalton)and three minor tsands.The virlon has 6 polypoptidesranging from 12600 to 33000 Daltons,The protein from LxCPV is rich in aslxirtic acid,glutamic acidand tyrosine,but poorer in cystein and methionine; The ratio of basic amino to acidic amino is l.43.By the stability test of the LxCPV RNA to RNase ,DNase and formaldehyde,the staining reaction ofacridine orange,the RNA is demontrated as double-strand RNA(dsRNA)with a Tm of 87℃.TheRNA genome consists of ten molecular mass from 2.46 to 0.35 MD,and with a totol estimated moleeular mass of l4.42 MD,Three distributive peaks of LxCPV RNA segments contour length are foundat 0.5μm,1.0μm and l.3μm respectlvely by electron micrcacopic analysis.

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