Study of carbon agglutination test for the rapid detection of respiratory syncytial virus

DONG Ji-Hua; JIANG Han-Zhen; ZHOU Sheng-Hua; XIE Hong-Bin; TIAN Mu-Zhen

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April 1996

DONG Ji-Hua, JIANG Han-Zhen, ZHOU Sheng-Hua, XIE Hong-Bin and TIAN Mu-Zhen. Study of carbon agglutination test for the rapid detection of respiratory syncytial virus[J]. Virologica Sinica, 1996, 11(2).

Citation: DONG Ji-Hua, JIANG Han-Zhen, ZHOU Sheng-Hua, XIE Hong-Bin, TIAN Mu-Zhen. Study of carbon agglutination test for the rapid detection of respiratory syncytial virus .VIROLOGICA SINICA, 1996, 11(2) : 116.

炭凝集试验快速检测呼吸道合胞病毒的研究

1.
同济医科大学附属协和医院

摘要

用炭凝集试验（ＣＡＴ）检测呼吸道合胞病毒（ＲＳＶ），结果表明该法是一种简便、快速、特异的诊断方法。用ＣＡＴ对１６株ＲＳＶ和８株其它病毒做试验，结果仅ＲＳＶ凝集，而其它病毒均阴性。用该法与细胞培养法检测８３份临床呼吸道感染幼儿鼻咽吸出物，结果ＣＡＴ法阳性率为６９．８８％（５３／８３），细胞培养法为３９．７５％（３３／８３），两者阳性检出率相差极显著。阻断试验证明ＣＡＴ是高度特异的。结果证明ＣＡＴ具有较高的敏感性与特异性，可用于临床ＲＳＶ标本的快速检测。
- 炭凝集试验单克隆抗体呼吸道合胞病毒抗原检测

Study of carbon agglutination test for the rapid detection of respiratory syncytial virus

Abstract

This study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal secretions from children with suspected RSV iofection.Of 83 specimens,53(69.88%)were positive by CAT,33(39.75%)were positive by virus culture,There were verysignificant difference in the positive rates beween CAT and culture Blocking test showed thatCAT has high specificity.We believe that CAT has a high sensitivity and specificity and it can beused for the rapid detection of clinical specimens of RSV.
- Carbon agglutination test Monoclonal antibody Respiratory Syncytial Virus Anti-gen detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of carbon agglutination test for the rapid detection of respiratory syncytial virus

Abstract: This study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal secretions from children with suspected RSV iofection.Of 83 specimens,53(69.88%)were positive by CAT,33(39.75%)were positive by virus culture,There were verysignificant difference in the positive rates beween CAT and culture Blocking test showed thatCAT has high specificity.We believe that CAT has a high sensitivity and specificity and it can beused for the rapid detection of clinical specimens of RSV.