Study on ELISA method for detecting specific circulating immune complexes in 
sera of patients with HFRS

ZHANG Dong-Hai; SUN Hui; GAO Feng

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August 1996

ZHANG Dong-Hai, SUN Hui and GAO Feng. Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS[J]. Virologica Sinica, 1996, 11(4).

Citation: ZHANG Dong-Hai, SUN Hui, GAO Feng. Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS .VIROLOGICA SINICA, 1996, 11(4) : 369.

检测肾综合征出血热抗原特异性循环免疫复合物ELISA方法的建立

1.
山东省淄博第二卫生学校

摘要

检测肾综合征出血热抗原特异性循环免疫复合物ＥＬＩＳＡ方法的建立张东海，孙辉，高峰（山东省淄博第二卫生学校，淄博２５５０１５）关键词肾综合征出血热病毒，循坏免疫复合物，特异性，ＥＬＩＳＡ肾综合征出血热（ＨＦＲＳ）发病机理有多种学说，其中在体液免疫学说中...
- 肾综合征出血热病毒循坏免疫复合物特异性 ELISA

Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS

1.
Zibo Second Health School，Zibo，Shandong Province 255015

Abstract

new ELISA method was established for detecting specific IgG,IgM,IgA type circulatingimmune complexes(CIC)in sera of patients with Hemorrhagic Fever with Renal Sydrome(HFRS).The rabbit anti-HFRS virus antibody was used as coated antibody and anti-humanIgG,IgM,IgA monoclonal antibodies were used as HRP conjugates.The results showed that thedetected rates of specific IgG,IgM,IgA types CIC in 73 acute phases(1￣8days after illness)were 69.49%,69.86% and 56.16% respectively,in 54 convalescence phases(10￣28 days afterillness)were 90.74%,85.19% and 75.93% respectively,28 sera in 1￣3 years after illness wereall 0.00%,36 serum specimens from normal and hepatitis B patients were all negative.The developed method showed highl specificity and sensitirity as well as reproducibility(CV:IGG12.07%,IgM6.29% and IgA12.89%)for the detection of HFRS specific CIC.
- Hemorrhagic Fever with Renal Sydrome（HFRS） Specific Circulating Inunune Complexes（CIC） ELISA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS

1. Zibo Second Health School，Zibo，Shandong Province 255015

Abstract: new ELISA method was established for detecting specific IgG,IgM,IgA type circulatingimmune complexes(CIC)in sera of patients with Hemorrhagic Fever with Renal Sydrome(HFRS).The rabbit anti-HFRS virus antibody was used as coated antibody and anti-humanIgG,IgM,IgA monoclonal antibodies were used as HRP conjugates.The results showed that thedetected rates of specific IgG,IgM,IgA types CIC in 73 acute phases(1￣8days after illness)were 69.49%,69.86% and 56.16% respectively,in 54 convalescence phases(10￣28 days afterillness)were 90.74%,85.19% and 75.93% respectively,28 sera in 1￣3 years after illness wereall 0.00%,36 serum specimens from normal and hepatitis B patients were all negative.The developed method showed highl specificity and sensitirity as well as reproducibility(CV:IGG12.07%,IgM6.29% and IgA12.89%)for the detection of HFRS specific CIC.