Morphology and Its M orphogenesis of the SARS-CoV Hb Strain in vitro

ZHANG Jia—min; ZHENG Cong．yi; HU Yuanyang*

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August 2003

ZHANG Jia—min, ZHENG Cong．yi and HU Yuanyang*. Morphology and Its M orphogenesis of the SARS-CoV Hb Strain in vitro[J]. Virologica Sinica, 2003, 18(4): 404-406.

Citation: ZHANG Jia—min, ZHENG Cong．yi, HU Yuanyang*. Morphology and Its M orphogenesis of the SARS-CoV Hb Strain in vitro .VIROLOGICA SINICA, 2003, 18(4) : 404-406.

SARS病毒的形态结构及其在体外培养细胞中的感染装配

1.
武汉大学生命科学学院，湖北武汉，430072

摘要

人冠状病毒1965年由Tyrrell等从人胎儿器官培养(HET．OC)中最初分离。其后Hamre和Kapikian 等从组织培养中分离了病毒l4j。2003年4月，世界卫生组织正式确认，引起这次非典型肺炎(国外称为重症急性呼吸综合症，Severe Acute Respiratory Syndrome，SARS)的病原体是冠状病毒的一个变种 SARS．CoV，以前没有在人体内发现过。继加拿大和美国之后，中国科学院北京基因组研究所和军事医学科学院微生物流行病研究所完成了SARS病毒中国流行株的病毒全基因组核苷酸序列测定，证实该病毒的基因序列与此前发现的冠状病毒存在较大差异，属于一种新的冠状病毒L2J。本研究利用电子显微镜负染色技术和超薄切片技术．分析了2003 年从湖北SARS患者血清中分离的冠状病毒的形态结构及在其敏感的体外培养细胞中感染、复制、装配过程
- SARS病毒
- , 形态结构：病毒装配

Morphology and Its M orphogenesis of the SARS-CoV Hb Strain in vitro

1.
College ofLife Sciences,Wuhan University,Wll ln 430072，China

Abstract

Morphological characterization of purified SARS．associated virus(SARS-CoV)from Hubei patient was carried out by negative stain and ultrathin section electronmicroscopy．The spike of isolated SARS．CoV virus is shorter an d smaller than Human coronavirus．A large quan tity of SARS-CoV particles could be observed in the infected Vero cells．Th e process of infection，assembly an d morphogenesis was observed．
- SARS-associated virus(SARS-CoV)
- , Morphology

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Morphology and Its M orphogenesis of the SARS-CoV Hb Strain in vitro

1. College ofLife Sciences,Wuhan University,Wll ln 430072，China

Abstract: Morphological characterization of purified SARS．associated virus(SARS-CoV)from Hubei patient was carried out by negative stain and ultrathin section electronmicroscopy．The spike of isolated SARS．CoV virus is shorter an d smaller than Human coronavirus．A large quan tity of SARS-CoV particles could be observed in the infected Vero cells．Th e process of infection，assembly an d morphogenesis was observed．