Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

LIU Yan-hua; FAN Wei-xing; WANG Xi-le; LIU Wen-qiang; XU Chuan-tian; ZHANG Zhi

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August 2004

LIU Yan-hua, FAN Wei-xing, WANG Xi-le, LIU Wen-qiang, XU Chuan-tian and ZHANG Zhi. Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain[J]. Virologica Sinica, 2004, 19(4): 353-355.

Citation: LIU Yan-hua, FAN Wei-xing, WANG Xi-le, LIU Wen-qiang, XU Chuan-tian, ZHANG Zhi. Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain .VIROLOGICA SINICA, 2004, 19(4) : 353-355.

猪细小病毒SD-68株NS1基因的克隆与序列分析

刘艳华 ¹ ,
范伟兴 ^1,2*,, ,
王锡乐 ¹ ,
刘文强 ¹ ,
许传田 ¹ ,

1.
1.山东农业大学动物科技学院，山东泰安 271018
2.
国家动物流行病学中心，山东青岛 266032

通讯作者： 范伟兴, ;

摘要

对猪细小病毒(PPV)SD-68株NS1基因进行了克隆和序列测定，结果表明SD-68株NS1基因全长1989bp，编码662个氨基酸组成的多肽。该序列与PPV SY-99株、Kresse株、NADL-2(5075)和NADL-2(4973)株的NS1基因比较，核苷酸的同源性分别为99.9%、99.9%、99.7%、98.1%，氨基酸的同源性分别为99.7%、99.5%、99.5%、96.7%。PPV SD-68株与MVM(i)、MEV(Abashiri)、CPV、FPV、BPV3、GPV NS1的进化树分析表明：PPV SD-68株NS1与MVM(i) NS1亲缘关系最近，与BPV3 NS1的亲缘关系最远；在Thr435和Ser473位点PPV SD-68株与MVM(i)完全一致，表明Thr435和Ser473是PPV SD-68株NS1潜在的磷酸化位点。
- 猪细小病毒SD-68株
- , NS1基因
- , 进化树

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

1.
1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
2.
China Animal Epizooti- ology Center, Qingdao 266032, China

Corresponding author: FAN Wei-xing,

Abstract

NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5%, 96.7% identity of amino acids with that of SY-99 strain, Kresse strain, NADL-2(5075) strain and NADL-2(4973) strain, respectively. The distances of NS1 gene of all strains analyzed by phylogenetic tree, we can conclude that SD-68 strain is near to MVM(i) strain and far to BPV3 strains. The residues of Thr and Ser at the position 435 and 473 in the NS1 gene of SD-68 strain are phosphorylated sites because they are also found at the same position in the NS1 gene of MVM(i) strain.
- Porcine parvovirus(PPV)SD-68 strain
- , NS1 gene
- , Phylogenetic tree

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

Corresponding author: FAN Wei-xing,

1. 1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
2. China Animal Epizooti- ology Center, Qingdao 266032, China

Abstract: NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5%, 96.7% identity of amino acids with that of SY-99 strain, Kresse strain, NADL-2(5075) strain and NADL-2(4973) strain, respectively. The distances of NS1 gene of all strains analyzed by phylogenetic tree, we can conclude that SD-68 strain is near to MVM(i) strain and far to BPV3 strains. The residues of Thr and Ser at the position 435 and 473 in the NS1 gene of SD-68 strain are phosphorylated sites because they are also found at the same position in the NS1 gene of MVM(i) strain.