Immune Response Induced by Recombinant Adenoviruses Expressing Serotype G2 or G3 VP7 of  Group A Human Rotavirus in Mice

ZHANG Cai-hong; JIANG Xiu-li; WANG Min; ZHANG Yan-ming; WANG Jian-wei; HONG Tao

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August 2005

ZHANG Cai-hong, JIANG Xiu-li, WANG Min, ZHANG Yan-ming, WANG Jian-wei and HONG Tao. Immune Response Induced by Recombinant Adenoviruses Expressing Serotype G2 or G3 VP7 of Group A Human Rotavirus in Mice[J]. Virologica Sinica, 2005, 20(4): 352-356.

Citation: ZHANG Cai-hong, JIANG Xiu-li, WANG Min, ZHANG Yan-ming, WANG Jian-wei, HONG Tao. Immune Response Induced by Recombinant Adenoviruses Expressing Serotype G2 or G3 VP7 of Group A Human Rotavirus in Mice .VIROLOGICA SINICA, 2005, 20(4) : 352-356.

表达轮状病毒G2和G3型vp7基因重组腺病毒的免疫效果

张彩红 ^1,2 ,
姜秀丽 ² ,
王敏 ² ,
张彦明 ¹ ,
王健伟 ^2* ,
洪涛 ²

1.
1.陕西杨凌西北农林科技大学 2.中国疾病预防控制中心病毒病预防控制所北京

摘要

为探索以非复制型腺病毒为表达载体的多价轮状病毒(Rotavirus,RV)基因工程疫苗的可行性,在前期工作的基础上,对表达我国G2和G3型RV流行毒株vp7基因的重组腺病毒的免疫效果进行了研究。分别用表达G2和G3型vp7基因的重组腺病毒rvAdG2VP7、rvAdG3VP7经滴鼻和灌胃两种途径免疫Balb/c小鼠,对免疫后小鼠的血清抗体、黏膜抗体和相关的细胞因子水平进行了检测和比较。结果表明,用表达G2和G3型vp7基因的重组腺病毒经滴鼻和灌胃两种途径免疫小鼠后,均可诱导机体产生较强的RV特异性免疫反应,包括体液免疫、细胞免疫和黏膜免疫,并能产生中和抗体。但免疫反应以Th2类为主,Th1类反应也占有相当的比例。本研究为新型RV基因工程疫苗的深入研究奠定了基础。
- 轮状病毒
- , vp7基因
- , 重组腺病毒
- , 基因工程疫
- , 免疫效果

Immune Response Induced by Recombinant Adenoviruses Expressing Serotype G2 or G3 VP7 of Group A Human Rotavirus in Mice

Abstract

In order to clarify the feasibility of adenovirus vector-based multivalent genetic engineering vaccine of Rotavirus (RV), we investigated the immune responses induced by the adenoviruses expressing serotype G2 or G3 VP7 of group A rotavirus on the basis of our previous reports. Balb/c mice were immunized with recombinant adenovirus rvAdG2VP7 or rvAdG3VP7, which expressing G2 or G3 type VP7,respectively, via intranasal or oral route for 3 times and serum antibodies, mucosal antibodies as well as the level of related cytokines were determinated. The results demonstrated that immunizing with the adenoviruses expressing serotype G2 or G3 RV VP7 intranasally or orally could induce strong rotavirus-specific immune response in mice, including humoral immunity, cell-mediated immunity, mucosal immunity as well as protective neutralizing antibody. The results also implied that Th2-like response triggered by the immunization is probably the primary response compared to Th1-like response. In summary, this study laid a fo
- Rotavirus vp7 gene
- , Adenovirus vector
- , Genetic engineering vaccine
- , Immune response

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Immune Response Induced by Recombinant Adenoviruses Expressing Serotype G2 or G3 VP7 of Group A Human Rotavirus in Mice

Abstract: In order to clarify the feasibility of adenovirus vector-based multivalent genetic engineering vaccine of Rotavirus (RV), we investigated the immune responses induced by the adenoviruses expressing serotype G2 or G3 VP7 of group A rotavirus on the basis of our previous reports. Balb/c mice were immunized with recombinant adenovirus rvAdG2VP7 or rvAdG3VP7, which expressing G2 or G3 type VP7,respectively, via intranasal or oral route for 3 times and serum antibodies, mucosal antibodies as well as the level of related cytokines were determinated. The results demonstrated that immunizing with the adenoviruses expressing serotype G2 or G3 RV VP7 intranasally or orally could induce strong rotavirus-specific immune response in mice, including humoral immunity, cell-mediated immunity, mucosal immunity as well as protective neutralizing antibody. The results also implied that Th2-like response triggered by the immunization is probably the primary response compared to Th1-like response. In summary, this study laid a fo