1992 Vol.07(2)
Up to present, few was reported about the natural infection of murineeytomegalovirus(MCMV) in laboratory mice. Based on the experimental infection of MCMV, we made a survey on the natural infection of MCMV in 10 common used strains of mice It was found that of 334 samples of submaxllary gland homogenate, none were positive for viral isolation, whereas specific anti-MCMV antibodies were found in 10.9-87.50% of different strains of mice. There were tendencies of increase in antibody positive rates as the mice...
Effect of Astragalus Membranaceus(AM) in lymphocyte subsets in an animal model of viral myocarditis were investigated by antigen specific cytotoxicity assay, The percentage of Thyl+cell in the peripheral blood and spleen was significantly higher in AM treated mice than in normal saline(NS) treated mice on 7th, 9th, 14th day(P<0.05) after Coxsackie B3 viral(CB 3V) inoculation, while it was statistically lower in myocardium in AM treated mice on 14th day after infection The percentage of L3T4+cell was mark...
Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of 27—34nm virus-like particles(VLPs) in cell cultures were visualized by immunoelectronmicroseopy(IEM). 2 of 3 rhesus monkeys inoculated with 20ml of pool of cell cultures from 4 to 7 passages developed significant eleva...
The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means of ABC-ELISA increased by 42.42% over the one by using conventional ELISA. Repeated experiments showed good reproductivity. Alternative tests using various antigens and antibodies of HBV and blockage test using p...
Half and one year after the epidemic of acute hemorrhagic conjunctivitis(AHC) caused by eoxsackievirus A24 variant(CA24v) in the autumn of 1988, 423 sera were collected and the neutralization antibody(NA) and specific IgM against CA24v were tested in microplate with Hela cells. The positive rate of NA was 37.9% in the spring and 48.8% in the autumn of 1989. The specific IgM was 3.3% in the spring and 6.7% in the autumn of 1989 and one strain of CA24v was isolated from a cold-like patient at this time. The r...
A sensitive and simplified method of ~(125)I-labeled peptide mapping was used to estimate the rates of the haemagglutinin(HA) mutation of 5 strains of influenza Al viruses and 5 strains of influenza A3 viruses. It could be calculated that the average rates of HA mutation for these two subtypes of viruses are 0.47% and 0.44% per year respectively. The strains isolated in 1986 showed some specialities as compared with the average rates of HA mutation The relation between the rates of HA mutation and the epide...
Serotypic classification of 14 HFRS virus isolates from different sources were studied using plaque reduction tests(PRNT) sera of rabbits inoculated with different HFRS viruses were tested against reference type-1 Hantaan virus and type-2 Seoul virus. The results indicated that except one strain(K_(24)), all the 13 isolates could be classified into either serotype 1 or serotype 2 according to the differences(≥4 fold) of the neutralizing titers between the sera reacted against reference type 1 and type 2 vir...
A RNA hyhridization assay for hepatitis D virus in serum and liver specimens, from human and infected chimpanzee experimentally, was described using a nonradioactive Digoxigenin labelled RNA probe, and was compared with the ~(32)P labelled RNA probe. Findings show that the sensitivity and specificity of the two probes are the same(about 0.2pg of homologous DNA). The results indicated that Digoxigenin labelled RNA probe can be used for efficacious detection of HDV RNA in serum and liver tissue from human or ...
In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the infected and mock-infected cell lysates were separated by diseontinous SDS-PAGE and transferred electrophoretically onto NC sheet. The blots were then probed with 12 kinds of biotinylated leetius(Con A, PSA, LCA, SBA, D...
In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...
The BALB/C mice were immunized with HeLa cells infected with respiratory syncytial virus(RSV, Wuhan strain). The immunized spleen cells were fused with SP2/0 myeloma cells which were obtained from the SP2/0 solid tumor grown in BALB/C mice. Thirteen hybridoma cell lines secreting antiRSV monoclonal antibody(MeAb) were established. The chromosome number of these cell lines was ranged from 94—104 and the seerated MeAbs belonged to routine IgG1, IgG2a, IgG2b subclasses. The ascites antibody titer was from 1: 1...
Recently, we isolated a non-occluded virus from spontaneous mortal Periplaneta fuliginosa(Serville). The virus suspension was mixed in sterile food to feed healthy P. fuliginosa nymphs(of 154—169 days). The percent mortality of the nymphs which died with infected virus was over 98%. Under electronmicroscope the virus was isometric particle about 23nm in diameter. The ultraviolet absorption of the virus was as character as nucleoprotein Purified virus suspension was used to distinguish the nucleic acid kinds...
It had been manifested that corn was readily infected by wheat BYDV The symptoms were similar to those of the wheat The most effective veetor aphid species was R. padi, while M. avena, S. graminnm and R. maidis were also among the vectors. The isolates belongs to the main strain of BYDV, eg. S. graminum/R. padi(GPV) strain. Mutual transmission in the fields between wheat and corn was evident.
In Zhejiang, Jiangsu and other provinces, 5 varieties of the garlic crops are totally diseased. The virus disease frequently causes degeneration of viruliferousness, loss of yields and reduction of tolerant to store. Symptoms due to the infection of virus are chlorotic mottling between the veins, yellow streaking and mosaic, scarcely visible and leaf distortion or deformation. The virus can be artificially inoculated on Vicia faba, then produces brown local lesions, slight spot on Chenopodium murale, necrot...
An immuno-colloidal gold labelling procedure was developed to localize barley mild mosaic virus(BaMMV) in thin sections of barley leaf and root tissues embedded in L. R. White resin, The optimal procedure for specific labelling of virus with a minimum of background labelling and economical use of reagents was to treat sections with buffer containing bovine serum albumen to block non-specific binding sites, treat with BaMMV-IgG(1/1000; 15 rain at room temperature), wash and label with goat anti-rabbit IgG-go...
During 1986—1989, 111 isolates of cucumber mosaic virus were obtained from tomato fields in Shandong province According to the host ranges and symptoms on different hosts, three pathogen strains of CMV were established as tomato fern leaf strain(CMV-ToF), tomato mosaic strain(CMV-ToM) and tomato latent(light mosaic) strain(CMV-ToL). There were some difference not only in bio-charaeteristics, but also in electrophoretic mobility and morphology of purified virus particle, molecular weight of capsid polypeptid...
Three strains of retrovius, which can give positive results both by ELISA and immunofluoreseenee assay(IFA) with SRV-1 newly have been isolated from Rhesus monkeys(Macaca mulatta) catehed frim three areas of China. It have been confirmed by Western Blot that their seroantibodies response with SRV-1 positively. One of them occurs cross-response with of SRV-1 and STLV. Lots of the virus particles having typical ultrastuctures of retrovirus have been observed by electron microscope.
With serotype B271 of Corynebacterium crenatum phage as a model, we established a method for studying the survival of aerosol of fine biological particles such as virus. The paper discussed some important techniques for the study of survival of viral aerosols, including the viral resistance to aerosolization, the size distribution of the particles of viral aerosols, caculation of physical decay with aerosol tracer and recovery technique for viral aerosol. It provided a general guide for the study of the sur...
This paper reports the derivation and calculation of several formulas for triangulation number and eapsomer number, The formulas are P=h~2+hk+k~2, T=pf~2, C=10T+2 and C=10p(n-1)~2+2. And the correlations among the formulas and the meaning of the parameters are given.
