1992 Vol.07(1)
Co-carcinogensis of seminal plasma(S), culture fluid of anaerobic bacteria(B) and Herpes Simplex Virus type 2(HSV-2) induced cervical carcinoma inmice were studied. The results showed that there were slight displasia insingle seminal Plasma(S) group or culture fluid of anaerobic bacteria group,cervical carcinoma could be induced by SB with an induction rate of 11.5%;23.1% of the carcinoma rate in HSV-2 group and 50.0% of the rate in HSV-2+SB group. The experimental results indicated that SB was both carcino...
We have demonstrated the constitutive polypeptides (PP) of Dane parti-cles employing SDS-PAGE Western Blot assay, investigated the antibody res-ponse ability to those PP in the HBV infected subjects in comparison withother HBV serum markers. The results suggest that there may be 12 bands inthe PAGE-graph of Dane particles. The Western blot confirmed that 6 PPs(P_(24) P_(27) P_(32) P_(36) P_(39) P_(42)) derived from S-ORF and 1(P_(21)) bearing core ande determinants was associated with C-ORF P_(24-25) could ...
We introduce 34 patients with anti-HBe Positive chronic hepatiti B (CHB). They accounted for 38% of all anti-HBe positive patients in the periodof the same time. There are 2-4 times reactivation in the period of 3-9 years(added up 80 times). During their reactivation of hepatits the clinical man-ifestation and degree of injured liver function are similar to HBeAg positivepatients in the same period. However, the ratio of occuring cirrhosis andhepatocarcinoma of anti-HBe positive CHB is significantly higher ...
Serotypic classification of 22 strain EHF viruses isolated from differenthosts and serotypic diagnosis of EHF patients in different endemic areas ofEHF were studied by using micro-CPE neutralization test (MCPENT) assay.The results of 13 strains classified as serotype 1 and 8 strains as serotype 2 byMCPENT assay, showed completely consistent with the results tested by pla-que reduction neutralization test (PRNT) method. The one neutralizing antigenbroad stnain Gou3 tested by PRNT was proved by testing with M...
A study on the inhibitory activities of traditional Chinese medicine "An-tipyretic I" oral liquor against 4 kinds of viruses was carried out in chickenembryo and two cell cultures using CPE inhibition method and haemagglutina-tion assay. The results obtained indicated that "Antipyretic I" oral liquor atdilution titer higher than 1/2 (the original concentration nf "Antipyretic I"was 245g dried medicinal herbs per 100ml) did not appear to be toxic tochicken embryoes and cell cultures and that "Antipyretic I" ...
In this study, we assayed the antiviral effect of AM and RG againstCoxsackie B-3 and Echo-19 viruses and the influence of these two drugs onthe susceptibility of human heart cells to viral infection. Results of the studyrevealed the following: human heart cells, depleted endothelial cells and fibro-blasts by 2 hours adsorption, grown up to monolayer by 9th-10th day, survi-ved more than one month. AM and RG have no direct inactivating effect onCox B-3 and Echo-19 viruses. Heart cells pretreated with AM and R...
For studying the function of Poliovirus 2A proteinaes in Polio-Vaccinarecombinant virus, We constructed the various chimeric virus of Polio-Vac-cinia, and obtained 4 Polio--Vaccinia recombinant viruses which contain var-ious fragments of Polio virus I type mahoney strain gene. These fragmentswere inserted into the TK region of vaccinia gene. The antigen of Polioviruswas expressed in these 4 recombinant viruses, the antibody against Poliovirusl type was induced in the rabbit infected by these chimeric viruse...
Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-...
The serological reactivity of erythromelalgia-related poxvirus (ERPV)which originally was isolated from the throab swab of a patient with eryhth-romelalgia was analyzed in the present study. Rabbits immunized by foot-padinoculation of ERPV started to produce neutralizing (NT) antibody to ERPVat 2 weeks postinfection (p. i.). The peak NT antibody titers were observedat 4 weeks p. i. and then began to decline. When eight antisera including sheepanti-Orf, bovine anti-parapox, rabbit antivaccinia, rhesus monkey...
Detection of the sequence related to E6/E7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E6/E7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E6/E7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and u...
The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.
A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant differenceranging from 0.47 to 2.45μ and 1.1μ in average in diameter. Virions werespherical in shape with six spikes Iocated and icosahedral vertices, measuring60nm in diameter. Magnifying the photograph, the subunit arrangement...
The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA EcoRI-I as the probe. For sequencing the polyhedringene, the KpnI-I fragment was cloned into M13mp18 vector.
Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...
A isometric virus-like particles(VLPs), which are approximately 34nm indiameter containing ss-RNA, have been purified from abnormal mycelial ofshiitake mushroom Leutinus edodes (Berk.) Sing. The SDS-polyacrylamide gelelectrophoresis demonstrated that the virions contain a single capsid polypeptideband with molecular weight about 22000 dalton. The nucleic acid extractedfrom purified preparations of 34nm VLPs showed only one band with mole-cular weight approximately 2.3810~6 dalton on the 1.5% agarose gel. T...
The complete encoding sequence with the initiation and stop codons ofMarek s disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I and the wild type AcNPV DNA. A recombinant baculovirus cloneBP38 I, which was expressing the MDV pp38 gene, was screened with theanti-MDV monoclonal antibody (MAb) H19 in fluorescence antibody test.Immunoblot indic...
