1993 Vol.08(2)
We have found that HEPES could induce and enhance the CPE caused by HFRS viruses in cultured Vero-E_6 cells. The infected cells were treated with 10-100mmol/L HEPES at 2—8 days post inoculation, several hours later, polykaryocytoses, networks and vacuoles appeared in the cell cultures. It could be seen under microscope without staining. On the contrast, no CPE was found in the untreated infective Vero-E_6 control cells or treated uninfected control cells The Vero-E_6 cells infected with 76-118, L_(99), HB_(...
Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of dengue virus 2 genome. Two primers specific for dengue virus 2 and conservative between geographical variants bracketed a 150 nucleotide base in the E gene, corresponding to bases 1437—1586 in the dengue 2 genomic RNA sequence. After digested with Hind Ⅲ, the products of the reaction was divided into two fragments, 111bp and 39bp, respectively. The amplified products and digested fragments were shown in the ethidium ...
The amplifying target sequence for Polymerase Chain Reaction(PCR) was chosen within the Internal Repeat Ⅰ (IR Ⅰ) of EBV genome. We used PCR of the primers to detect 31 biopsied specimens of different disease and 4 nasopharyngeal epithelial cultures. EBV genomes were detected in differentiated nasopharyngeal carcinoma(NPC)biopsies 5/5, poorly differentiated NPC 4/4, Hodgkin s Disease (HD) 3/5, non-Hodgkin s Disease (NHD) 0/2. Other head and neck tumors 1/6, chronic inflammatory nasopharyngeal 0/5, normal nas...
Clinical uses of garlic preparations in the prevention and treatment of Human Cytomegalovirus (HCMV) infections are effective. On the basis of this, we screened a series of garlic components or compositions for their anti-HCMV activities in vitro by employing cytopathic effect inhibiting test and plaque reduction assay. It is confirmed that garlic contains more than one components, at least including Allitridi and Adjone derivatives, which have dominant anti-HCMV action. Further researches on the anti-viral...
The study on the inhibitory activities of Black Tea, Blue Tea and Dark Tea against Rotavirus was carried out in cell culture by using CPE inhibition method, The experimental results indicated that both the Black Tea liquor and the Blue Tea liquor at dilution titer 1/1000(40μg of the tea at 1 ml cell culture solution) and the Dark Tea liquor at dilution titer 1/100 had rather strong inhibitory activities against the rotavirus. The results also showed that the tea liquors at dilution titer 1/10(4 mg of the te...
In the present work, the primers HIV-1 Pr1 and HIV-1 Pr2 were synthesized in which the EcoRI and Hind Ⅲ recognition sites as well as the termination codons were designed for convenience of subsequent cloning and expression Micrograms of target gene sequence was produced by 30 rounds of amplification with pJG423 as a template The amplified gene was digested with both EcoRI and Hind Ⅲ and ligated to pUC19 predigested with the same restriction endonucleases. The recombinant plasmid pUPR1 nd pUPR2 were identifi...
The morphology of Oxyplax ochracea (Moore) Nuclear Polyhedrosis Virus (OoNPV) isolated from naturally infected larvae is described in this paper. The endonuclease digestion map of OoNPV-DNA is obtained by restriction endonucleases EcoRI and HindⅢ digesting, The average molecular weight of OoNPV is 102.0919~6d, i.e. 147.77kb.
A sensitive indirect dot-ELISA was developed by using nitrocellulose membrance (NCM) instead of microplate, 3% casin as a blocking agent, horse-radish peroxidase conjugated protein A as an enzyme-labelled complex and a dilution by 100 folds of IgG from antiserum of rabbit to PRV-Ys strain as the working concentration. and was found to b workable for assessing an amount of PRV particles at ng level (ave.10ng/spot, at 37℃). A PRV-RNA cDNA molecular probe (the insert ca. 300bp) was successfully constructed wit...
Through sereral years of experiments and researches it is shown that the transmission potential of BYDV by Rhopalosiphura padi has been drastically enhanced, This fact enabled the expansion and development of this disease from the arid and semiarid areas in the north toward the more fertile wheat field at the irrigated areas and even toward the wheat areas in the south. In 1988 and 1989 it has been successively induced the prevalence of BYDV at western Guanzhong of Shanxi Province and in spring of 1989 at R...
The symptoms, virion morphologies and insect vectors of rice yellow stunt disease occurred in mainland China and riee transitory yellowing disease found in Taiwan are very similar or identical as deseribed in the previous reports, therefore they are putatively regarded as the same. But serological comparison between the causative viruses of two diseases has not been done so far. In this paper we report on the reactions of crude Rice Yellow Stunt Virus (RYSV) preparations extracted from diseased rice plants ...
Wheat soil-borne virus diseases in Ya an, Sichuan and Chang an, Shanxi (PRC) were caused by wheat spindle streak mosaic virus (WSSMV) and those in Anji and Xichang. Zhejiang and Yixing, Jiangsu were caused by complex of WSSMV and soil-borne whoat mosaic virus (SBWMV)infection Both viruses could co-infect the same wheat plant but independently distributed inside infected cells. Chinese WSSMV contained 2 species of RNA with molecular weight of 2.6×10~6 and 1.5×10~6, identical or similar to that of Japanese is...
Studies on grass carp hemorrhage virus (GCHV) genome transcription in vivo have been carried out Viral mRNA labeled with α-~(32)p ATP was extracted from infected cells treated with actinomycin D and detected by means of liquid phase hybridization and Northern blot hybridization. The results showed that the early and late stage mRNA were transcribed at 4hr and 8hr after infection and roughly divided into four groups, among which each one corresponds to the relative viral genome segment.
Lecithin was extracted by organic reagent from eggs and mixed with PxGV to infect 2th-3th instar Px larvae. Through the treatment of statistical method, we got three groups time-mortality regression lines, y=18.1x-59.8 (GV only), y=27.23x-99.85 (GV+30mg/ml lecithin) and y=13.6x-18 (GV+70mg/ml lecithin). Comparing with GV control group, mortality of GV+70mg/ml lecithin group increased by 17％ and LT50 decreased by 1.066 days This result shows lecithin can enhance the infection of PxGV.
A virus disease of sugar beet occurred in Ningxia showing symptoms of yellows, parch blight and necrotic yellow vein of foliage leaves. The rod shaped virus was isolated from sugar beet plants affected with rhizomania disease The virus particles had a width of about 20 nm and at lengths of 65—110nm, 270—300nm, and 390—420nm, The virus could infect Bete uulgaris L., Spinacea oleracea, Chenopodium quinoa, C. amaranticolor, Tetragonia expansa. The virus could react with BNYVV antiserum According to the above d...
Detection of HFRS virus infectivity titer using virus-infected cell enzyme-linked immunosorbent assay(VIC-ELISA) was more sensitive than the indirect "double-sandwich" ELISA and indirect immunofluorescent antibody(IFA) technique 10 and 100 times respectively. Detection of rabbit anti-HFRS virus antibody titer using VIC-ELISAwas more sensitive than the indirect "double-sandwich" ELISAand indirect IFA technique 1.6 and8 times respectively. The VIC-ELISA can be used for rapid, sensitive and efficient detection...
