Advances in Baculovirus Transpasable Elements
A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.
A total of 7.2 kb of full-length genome of hepatitis E virus (HEV)XT-179 strain was cloned as cDNAs that isolated from a HE patient s fecal sample during an epidemic outbreak peak of Sept. 1990 in the East Xinjiang of China, and the complete nucleotide sequence has been determined. This strain have a genome length of 7194 nucleotides followed by a poly(A)tail. The nucleotide composition of the HEV genomic RNA is 17.0% A,31.9%C,26.0%G and 25.1%U,conferring an overall G+C content of 57.9%. There are mine res...
Antisense phosphorothioate oligodeoxynueleotides respectively complementary to initial region of 2.1kb mRNA(oligomer A,14nts)and enhancer I(oligomer B,12nts)of HBV were synthesed by chemi cal synthetic method,and inhibition of these oligomers on expression of HBsAg and HBeAg in cell cul ture system was studied. The oligomer A inhibited more than 50% of HBsAg at 3mol/L, but the oligomer B did at 6mol/L. On the contrary,the oligomer B showed 35% inhibition on expression of HBeAg at 3mol/L,but 22% the oligo...
The levels of interleukin-1 and interleukin-2 production in 16 healthy blood donors bone mar row mononuclear cells infected by HBV in vitro and on 16 cases with chronic hepatitis B were simulta neously assayed. The evidence of virus growth is based on the detecion of HBcAg in bone marrow mononuclear cells by immunoperoxidase assay. It was found that HBcAg is positive in 50% (in vitro) and 43. 7%(in vivo) respectively (P0.05). The levels of interleukin-1 and interleukin-2 produetion are markedly decreas...
In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. Southern blot showes that RCN has TK gene sequence though it hybridizes week ly with vaccinia virus TK gene. But RCN is resistant to 5-BUdR even after it was plaque purified in HAT media.
This paper described immunization effect and response of two and three injections with 3ml inacti vated EHF vaccine in volunteers. This experiment was derided into three groups. Group 1. Injected three times on day 0,7 and 28(1 ml once);Group 2.Injected twices on day 0 and 28(1.5ml once); Group 3.Injected twice on day 0 and 28(2ml first, 1ml second). The results showed that side effect was not found locally and generally in three groups,and the neutralization antibody positive rate was 87.1%, 79.4 %and 76.7...
By detecting specific HSV nucleotide sequences with polymerase chain reaction (PCR), we investigated one hundred of pregnant women and women in childbirth for understanding HSV infection rate in their reproductive organs and the posibility of transmiting HSV to filial generation. The results showed the transmission rate of HSV from infected mother to filial generation was re spectively 11.1% and 54.5% by HSV diffusion vagina:for primary HSV and secondary HSV,the transmission rate was 66.7% and 29.4%,res...
By detecting specific HSV nucleotide sequences with polymerase chain reaction (PCR), we investigated one hundred of pregnant women and women in childbirth for understanding HSV infection rate in their reproductive organs and the posibility of transmiting HSV to filial generation. The results showed the transmission rate of HSV from infected mother to filial generation was re spectively 11.1% and 54.5% by HSV diffusion vagina:for primary HSV and secondary HSV,the transmission rate was 66.7% and 29.4%,res...
SDS-PAG electrophoresis showed that the polyhedron proteins of Leucaia separata nuclear polyhedrosis virus(LsNPV)was consisted of several polypeptides, the major band of MW 32kD was the major structural polypeptides of polyhedra. Amino acid composition of 32kD protein was analyzed after purification by chramatography of Sephacryl S-200 column. The cell growth of three tumor (Hela, HLAMP, HICAM)was inhibited by 32kD protein, DNA replication labeling with ~3H-TdR recognized the result.
Purified potato virus Y, X, and S were detected by indirect enzyme-linked immunosorbent assay (ELISA) on nitrocellulose membranes. Potato virus Y, X, and S in infected tobacco, sprouts of potato tubers, rose end of dormant tubers were detected respectively. The results indicated that the small amount of sample required permit the detection of as little as 2.76pg, PVY, 2.835pg PVX and 5.83pg PVS in purified preparations. PVX was detected in infected tobacco、rose end of dormant tubers and sprouts of potato tu...
A rice virus-like disease broke out in Wenzhou, Zhejiang Province. The infected rice plants became stunt with darking and hardening leaves. Along the veins of sheath, culm and underside of leaves, some waxy swelling could be found, white first, then black brown. When four insects (Laodelphax striatellus, Nelaparvata lugens, Sogatella furoifela and Nephotettix cincticeps)were tested as vector candidates, only the small brown plant hopper(L. striatellus)transmitted the disease in a persistant manner without...
Five virus free isolates of Polymyxa graminis were purified through sand culture of susceptible wheat cv Yangmai 4 inoculated with selected cystosori from diseased wheat roots which were collected from wheat spindle streak mosaic virus (WSSMV) infected fields. The virus free P. graminis acquired WSSMV by mean of inoculation of the cystosori to the roots of wheat plants bearing WSSMV symptom and then transmitted the virus to the healthy wheat plants after further inoculation. The four isolates of P. graminis...
The temperature-inducible expression vector containing the beet necrotic yellow vein virus(BNYVV)isolate NM coat protein gene was constructed and transferred into E. coli DH5 . High-level expression of the specific protein was achieved by temperature induction. The results of SDSPAGE and Western blotting show that the expression product which accumulates 19.5% of the total cellular proteins estimated by Shimadzu CS-910 scanning is 21kd BNYVV coat protein.
Tai MV was routinely detected and diagnosed by ELISA method. The detective sensitivity of the method was 4ng. Fields detective results showed that the soil, the roots tubers of Taizishen, symptomless plants and the surfaces of seeds had serological reactions with Tai MV antiserum. And it was confirmed that Taizishen seeds could eliminate Tai MV completely by infusing in 10% Na_3PO_4 solution for 20 minutes.