1990 Vol.05(4)
A semimicro quick monolayer plaque assay of viruses (SQMPAV) without preparing the monolayer in advance was developed. In this assay, the cells and viruses were simultaneously inoculated into the wells of multi-well tissue culture plates. So the attachment of cells and the adsorption of viruses occurred at the same time. Then, the plaques were produced as the monolayers were formed under the nutrient gel overlay. With the model of polioviruses, a series of studies on SQMPAV, including the experimental condi...
This paper reports the protective effect of human CSF-1 on human fibroblast cultures infected by viruses in vitro, that is, before virus challenge, the treatment of cell cultures with CSF-1 may result in the inhibition of virus replication and virus-induced cytopathy. The challenge viruses included 2 strains of HSV, 1 strain of influenza A_3 virus, 1 strain of ECHO-11 virus, 5 strains of different serotypes of adenovirus, 1 strain of rhinovirus and 1 strain of VSV. The activity of CSF-1 was specific, it was...
To explore the histopathological features in chronic hepatitis D, 5 out of 120 cases of HBsAg-positive liver samples were found to be intrahepatitic HDAg-positive by direct immunof luorescence and immunoperoxidase assays. Among 5 cases, 3 had active cirrhosis, 2 cases with chronic active hepatitis. Wellmatched cases with chronic hepatitis B were used as control, Emphasis was put on similarity and difference in histopathological findings between hepatitis D and hepatitis B by light and electron microscopy an...
A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...
Specific IgM from patients with epidemic parotitis ( EP ) was detected by enzyme linked immunosorbent assay (ELISA). The mumps virus antigen was prepared by using mumps virus Ender s strain. The detected IgM antibody was confirmed by 2-EM destruction. Sensitibity of the assay for mumps virus-IgM was high as showing a titer of 1:12800 in the positive sera from the patients. In clinically diagnosed 79 cases from the patients with epidemic parotitis, 72 cases were IgM positive, and the positive rate was 91%. I...
The extract of Monordica charantia (E. M. c.) was found to have a significant protective effect on Japanese B encephlitis virus infection in vivo. When mice were administered intraperitoneally with a dose of 0.4 mg E. M. c. at 18 hr, 12 hr, 4 hr before subcutaneous challenge with the virus respectively, the protective rate of treated group is 66%. E.M.c. was demonstrated as an interferon inducer. Type I IFN was found in rabbit serum and its level peaked at 2hr after intravenus injection of E. M.c. E.M.c. en...
Rabbits were inoculated with EHFV-H 114 strain isolated from urine of the patient with EHF in the acute phase. The duration of viremic stage of rabbits infected with EHFV-H114 was studied by means of immunoflourescent technique and culture assays. Specific flourescence for EHFV antigen was first observed in both monocytes and lymphocytes on the 6th day, the next day, also was seen in granulocytes, with the strongest antigen reaction on the 10-12th days, and dissappeared on the 15th day of experimental infec...
In this paper chronic hepatitis B and its virus carriers were treated with HB Vaccine and small doses of interferon and thymosin. After one course of interferon group, placenta peptide group and HB-Vaccine group, the negative percentage of HBsAg were 13.7%, 5.9% and 0%, respectively. The changes of HBeAg were 68.8%, 72.7% and 20%, respectively. The results showed that combination of interferon and immunologic stimulant can control the replication of Hepatitis B Virus, and impel HBeAg to change, but can not ...
This paper reported the research results for biological properties of six strains of Chikungunya virus (CHIKV) isolated from bats, mosquitoes and patients in Yunnan, China, and Ross strain of CHIKV isolated from Africa. These virus strains all could cause illness and death regularly in two to four-daysage sucking mice, produced evident CPE in the C6/36, BHK21, Vero and primary hamster kidney cells. These virus strains agglutinated red blood cells of dove, goose, chicken, duckling, sheep and human type O at ...
Effect of temperature on the growth dynamics of the nuclear polyhedrosis virus form Ectropis obliqua hypulina (EoNPV) in its host larvae was studied through polyhedra counting, counter immuno-electrophoresis, single immunodiffusion and rocket immuno-electrophoresis tests. The propagation of the virus in the host larvae fed with the NPV at the concentration of 2.6 10~7PIB/ml and reared at 26℃ was normal. The polyhedra titer or its relative equivalences increased positively with the time after inoculation and...
By using BsNPV DNA, HSV DNA and λDNA, the paper reported the re suits of the influence of ionic strength, pH value, reaction temperature and time on the labeling process of DNA with radioactive iodine in the presence of thallic chloride.When pH value was 4.8—4.9, ionic strength(Na~+)was 0.1mol/L, the temperature was 70℃ and the reaction time was 30 minutes, very high specific activity can be yielded. The percentage of ~(12)Ⅰ bound to DNA is 10—30% and the specific activity of DNA probe is over 10~8 cpm/μg D...
Gynura aurantiaca plants infected by citrus exocortis viroid (i.e. CEV) were applied in different concentrations of exogennous gibberellin (GA,5ppm, 10ppm, 50ppm, 100ppm, 200ppm), n(?)phthalen (Nal., 100ppm, 300ppm), 5-flurouracil (5-FU, 0.5mg/L, 1mg/L, 2mg/L) solutions respectively. It was found that the relative infectivity indexes of plants had to some extent been changed. Nucleic acids of young leaves were extracted and subjected to 5% bidirectional polyacrylamide gel electrophoresis (PAGE) followed by ...
The seed borne virus(TMV) in tomato can be disinfected by 1 day of dry heat treatment at 70℃, or by more than 4 years storage. Soaking the tomato seeds in the 10% of Na_3PO_4 solution for 30 minutes can not disinfect the virus entirely. However, the 3 methods mentioned above were not effective for the disinfection of seed-borne virus in pepper. It suggested that TMV pepper strain have more strong viability than the TWV strains in tomato.
Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of the DNA with the three restriction endonucleases, respectively, and three DNA libraries containing different restricted fragments were constructed. Photobiotin-labeled viral DNA and bovine thymus DNA were used as pr...
Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because the titers of two ascite McAbs were up to 1: 10~5 or above by indirect ELISA with RV of Bovine HN-7, BRV007, BRV014 and NCDV, Porcine Na86, Simian SA-11 and Human Wa strain, but were≤ 1: 8 and≤1: 100 respectively...
To compare relationship between two pre-s proteins and HBV replication HBV DNA in situ hybridization was applied to a group of patients with chronic hepatitis by bio-probe, in conjunction of detection of intrahepatic HBsAg, HBcAg, pre-s1 and pre-s2 antigens. It was found that the positive rate for pre-s1 was up to 75%(15/20), being significantly higher than that of pre-s2 (35%) in the same group. More importantly the presence of pre..sl, rather than pre-s2, was in parallel with the presence of intrahepatic ...
