2020年35卷2期

While host proteins incorporated into virions during viral budding from infected cell are known to play essential roles in multiple process of viral life cycle of progeny virus, these characteristics have been largely neglected in studies on rabies virus (RABV). In this issue, Zhang et al. performed proteomics studies of the RABV virions with good purity and integrity to define 49 cellular proteins incorporated into mature virions, and 24 of them were likely involved in virus replication according to the functional annotation analysis. Furthermore, Zhang et al. used cryo-electron microscopy (Cryo-EM) to observe the purified RABV virions, generating high-resolution pictures of RABV particles with unprecedented structure details. The cover image clearly displays an intact bullet-shaped structure with four distinct electron-dense layers consisting of glycoprotein spike, viral envelope, matrix protein helix and RNP complex from outside to inside. Please see page 143–155 for details.